Fig. 6From: Tyr724 phosphorylation of ELMO1 by Src is involved in cell spreading and migration via Rac1 activationELMO1 pY724 facilitates cell migration. a Trans-well migration assay. NIH3T3-Dock180-Control, −wild-type ELMO1, and -Y724F ELMO1 cells were suspended in DMEM without FBS were seeded into the upper chamber. DMEM containing 10 % FBS was added to the lower chamber. After 11 h, migrating cells were stained with 0.04 % crystal violet. Representative micrographs after incubation for 11 h are shown. b The motility of the individual cells was evaluated by measuring the migrating cells. The data are shown as mean ± SD. **, P < 0.005; ***, P < 0.0005Back to article page