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Fig. 1 | Cell Communication and Signaling

Fig. 1

From: Tyr724 phosphorylation of ELMO1 by Src is involved in cell spreading and migration via Rac1 activation

Fig. 1

Phosphorylation of ELMO1 by Src. a In vivo phosphorylation of ELMO1 by SFKs. HEK293T cells were transfected with pCXN2-Flag-ELMO1 in combination with plasmids expressing SFKs: Src, Fyn, Yes, Lck, Lyn, and Hck. Lysates from these cells were subjected to immunoprecipitation with anti-Flag antibody (Ab) for ELMO1, followed by immunoblotting with Ab to phosphotyrosine (PY). Anti-non-phospho-Src Y416 antibody recognizes conserved region of other SFKs such as Lyn, Fyn, Lck, Yes, and Hck. IP: immunoprecipitation, TCL: Total Cell Lysate, pELMO1: tyrosine phosphorylated ELMO1, pSFKs: phosphorylated SFKs. b In vitro phosphorylation of ELMO1 by Src. Recombinant proteins of c-Src and GST-ELMO1 were mixed with or without ATP for kinase reaction. Reaction samples were analyzed by immunoblotting with Abs against PY (upper) and GST (lower). Auto-pSrc: autophosphorylated Src

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Cell Communication and Signaling

ISSN: 1478-811X