Fig. 2
From: Accumulation of the PX domain mutant Frank-ter Haar syndrome protein Tks4 in aggresomes
Different localization patterns of Tks4 and Tks4R43W proteins in cells. 2a, COS7 cells were transiently transfected with V5-Tks4, V5-Tks4R43W or V5-empty constructs. After 18 h, cells were fixed and stained for Tks4 with anti-V5 antibody. 2b, The percentage of aggresomes in cells was quantified by counting 300 cells/sample by using ImageJ software. Error bars represent the standard deviation from three independent experiments. 2c, Tks4R43W aggresomes colocalize with the HDAC6 aggresome marker. COS7 cells were transiently transfected with V5-Tks4 or V5-Tks4R43W constructs. After 18 h, cells were fixed and stained stained for Tks4 (with anti-V5 antibody, red) and HDAC6 (green)