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Fig. 7 | Cell Communication and Signaling

Fig. 7

From: Bile acid effects are mediated by ATP release and purinergic signalling in exocrine pancreatic cells

Fig. 7

Effect of P2 receptor inhibitors on intracellular Ca2+ responses induced by CDCA in Capan-1 cells. a and b Representative recordings of [Ca2+]i transients in Capan-1 cells with or without P2 receptors inhibitors (standing bath). Cell were incubated with a mix of P2R inhibitors: PPADS (250 μM), suramin (250 μM), and 10 μM of AZ 10606120 and 10 μM of A438079 for 25 min. a, c The presence of P2R antagonists markedly inhibited [Ca2+]i response induced by ATP (100 μM), but had no effect on Thapsigargin (1 μM) induced Ca2+ response. b, d Incubation cells with P2R antagonists inhibited [Ca2+]i transient induced by 0.3 mM CDCA but not by Thapsigargin (1 μM). c, d Change in [Ca2+]i above baseline are given as mean values ± SEM of 7–15 cells per each independent experiment (n). Arrows indicate the time of adding the stimuli. *= P < 0.05, ***= P < 0.001, N.S = not significant

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