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Fig. 5 | Cell Communication and Signaling

Fig. 5

From: Bile acid effects are mediated by ATP release and purinergic signalling in exocrine pancreatic cells

Fig. 5

The acute and chronic effects of CDCA on intracellular ATP concentration in AR42J and Capan-1 cells. a Original trace for luminometric measurements of ATP based on Capan-1 measurement. Baseline values were recorded every 20 s for 2 min. Cells were then stimulated with 0.3 mM (Capan-1) or 0.5 mM (AR42J) with CDCA or with vehicle. Stimulated ATP release was recorded every 1 sec for 1 min directly after addition of CDCA, or after 12 min or 24 h of incubation. Finally, cells were permeabilized with digitonin (50 μM), added automatically using pump to release remaining ATP. Panel b and c show the values of released and remaining ATP in Capan-1 and AR42J cells, respectively. These values were calculated per cell after 1, 12 min and 24 h (n = 8, 7, 10 and n = 5, 5, 10) of incubation with CDCA. Data are shown as mean values ± SEM; ***= P < 0.001, N.S. - not significant

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