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Fig. 2 | Cell Communication and Signaling

Fig. 2

From: Bile acid effects are mediated by ATP release and purinergic signalling in exocrine pancreatic cells

Fig. 2

Sidedness of ATP release and the effect of vesicular and non-vesicular inhibitors on ATP release pathways. a Mean values of ATP released across basolateral (BL) and luminal (L) sides from Capan-1 cells after 1 min of apical or serosal stimulation with 0.3 mM CDCA. b Inhibitors of vesicular transport decreased ATP release from Capan-1 cells in response to 0.3 mM CDCA. Cells were incubated with vacuolar-type H+-ATPase inhibitor (bafilomycin A1, 1 μM; n = 5), vesicle fusion inhibitor N-Ethylmaleimide (NEM, 250 μM, n = 4) and brefeldin A (5 μg/ml; n = 8). c Influence of the non-vesicular transport inhibitors: gadolinium chloride (Gd3+, 50 μM; n = 6), probenecid (500 μM; n = 7), 10Panx - mimetic pannexin peptide (100 μM; n = 7), as well as P2X7 receptor inhibitors AZ10606120 (10 μM; n = 9) and A438079 (10 μM; n = 7) on CDCA-induced ATP release from Capan-1 cells is shown. Results are given as mean net values ± SEM. *= P < 0.05, **= P < 0.01, ***= P < 0.001

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