Figure 9

Palmitoylated claudin-7, MMP activity and CD147 (a) Zymography of HEK, HEK-EpC, HEK-cld7, HEK-EpC-cld7 and HEK-EpC^mAG-cld7 or HEK-EpC-cld7^mPalm supernatants. (b) HEK cells were seeded in matrigel-coated transwell plates in the presence of anti-CD147 (10 μg / ml) (a representative example is included in Figure 8a); invasion and penetration was evaluated as above; significant inhibition by anti-CD147: s. (c) Confocal microscopy of HEK-cld7, HEK-EpC-cld7 and HEK-EpC^mAG-cld7 or HEK-EpC-cld7^mPalm cells stained with anti-cld7 and anti-CD147 (single fluorescence and overlays, scale bar: 10 μm). (d) HEK-cld7, HEK-EpC-cld7, HEK-EpC^mAG-cld7 and HEK-EpC-cld7^mPalm lysates were precipitated with control IgG or anti-CD147; after SDS-PAGE and transfer precipitates were blotted with anti-EpC, anti-cld7 and anti-CD147. Palmitoylated cld7-promoted invasiveness likely is supported by associated MMP14 and CD147, which can strengthen MMP2 and MMP9 activation and expression.