Figure 6

Cooperativity of palmitoylated claudin-7 with the cytoskeleton (a) Cld7 co-localization with ezrin and src (confocal microscopy, green and red fluorescence and overlays, scale bar: 10 μm) and (b) control IgG and cld7 co-immunoprecipitation with ezrin and p-ezrin in the absence or presence of a palmitoylation inhibitor (2-BP, 15 μM) and (c) control IgG, cld7 and ezrin co-immunoprecipitation with cld7, EpC, CD104 and ezrin; EpC, CD49d and CD166 co-immunoprecipitation with cld7, ezrin and EpC; (d) cld7 co-immunoprecipitation with src, FAK, paxillin and cld7 in transfected HEK cells. Cld7 associates with the cytoskeletal linker protein ezrin. Cld7 palmitoylation facilitates ezrin binding (and F-actin formation). This also accounts for the association with CD49e, CD166, src, FAK and paxillin. Recruitment of the latter two towards cld7 does not essentially depend on, but becomes strengthened by cld7 palmitoylation.