Figure 2

Claudin7 palmitoylation and TJ formation (a) Flow cytometry analysis of claudin and ZO-1 expression in HEK cells; the mean fluorescence index (% stained cells x mean intensity of staining ± SD; three assays) and representative examples are shown; (b) colocalization of cld7 with cld3 and ZO-1 and of cld4 with ZO-1 (scale bar: 10 μm); (c) immunoprecipitation with control IgG, anti-cld7 and anti-ZO-1 and co-immunoprecipitation of cld7, EpC, cld3, cld4 and ZO-1 in HEK-EpC-cld7, HEK-EpC^mAG-cld7 and HEK-EpC-cld7^mPalm lysates. Palmitoylated cld7 poorly colocalizes and co-immunoprecipitates with TJ proteins.