Long-term culture and significant expansion of human Sertoli cells whilst maintaining stable global phenotype and AKT and SMAD1/5 activation

Table 1 The sequences of gene primers used for RT-PCR

Gene	Primer sequence		Product size (bp)	Tm(°C)
WT1	Forward	TGACTCTCCACTCCTCCTCAC	170	60
WT1	Reverse	ACCAACTCTTCCAGGCACAC	170	60
GATA4	Forward	GCCTCCTCTGCCTGGTAAT	210	60
GATA4	Reverse	CAGTCCCATCAGCGTGTAAA	210	60
GATA1	Forward	GAAACCGCAAGGCATCTG	140	60
GATA1	Reverse	CCCAGCCACCACCATAAAG	140	60
GDNF	Forward	TGAAACCAAGGAGGAACT	180	58
GDNF	Reverse	CACTCACCAGCCTTCTATT	180	58
BMP4	Forward	TTTGTTCAAGATTGGCTGTC	324	60
BMP4	Reverse	AGATCCCGCATGTAGTCC	324	60
SCF	Forward	GTCATTGTTGGATAAGCGAGAT	457	60
SCF	Reverse	ATGGCTGCCCAGTGTAGG	457	60
FGF2	Forward	TCCTTTCTCCCTCGTTTCTTC	195	55
FGF2	Reverse	GATGTTTCCCTCCAATGTTTC	195	55
EGF	Forward	CTGATATAGATGAATGTGAGATGGG	190	55
EGF	Reverse	GTGGAGTAGAGTCAAGACAG	190	55
FSHR	Forward	TCTGCTGGTTCTGTTTCA	215	58
FSHR	Reverse	CATTCCTTGGATGGGTGT	215	58
AR	Forward	CCTTCACCAATGTCAACTCC	197	60
AR	Reverse	CCACTGGAATAATGCTGAAGAG	197	60
ABP	Forward	GGCTTTACCAGGAGAAGAC	150	60
ABP	Reverse	CATGGCTTCTGTTCAGGG	150	60
ACTB	Forward	CGCACCACTGGCATTGTCAT	253	55
ACTB	Reverse	TTCTCCTTGATGTCACGCAC	253	55

ISSN: 1478-811X