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Figure 5 | Cell Communication and Signaling

Figure 5

From: Long-term culture and significant expansion of human Sertoli cells whilst maintaining stable global phenotype and AKT and SMAD1/5 activation

Figure 5

The expression of WT1, GATA4, GATA1, GDNF, BMP4, KITLG , FGF2 , EGF , FSHR , AR and ABP in human Sertoli cells after culture for P1, P5 and P10. (A, B) RT-PCR showed the transcripts of WT1, GATA4, GATA1, GDNF, BMP4, KITLG, FGF2, EGF, FSHR, AR and ABP in human Sertoli cells at P1, P5 and P10. ACTB was used as a loading control, and RNA without reverse transcriptase enzyme but with PCR of ACTB served as a negative control (NC). There was no significant difference (P >0.05) in the gene expression levels of WT1, GATA4, GATA1, GDNF, BMP4, KITLG, FGF2, EGF, FSHR, AR and ABP in human Sertoli cells after culture for P1, P5 and P10.

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