Morphology of the testes from OA patients and the isolation of human Sertoli cells from patients with OA. (A) H&E staining revealed the morphology of testicular tissues from OA patients. (B) Seminiferous tubules were obtained after the first enzymatic digestion with collagenase IV and DNase I. (C) The mixture of male germ cells and Sertoli cells was isolated from seminiferous tubules using the second enzymatic digestion. (D) Sertoli cells remained in the culture plates after differential plating with the removal of male germ cells. Scale bars in A-C = 100 μm; scale bar in D = 50 μm.