Skip to main content

Advertisement

Table 1 Specific interaction between PKCθ and Coronin 1A in the GAL4 two-hybrid system

From: Novel Protein kinase C θ: Coronin 1A complex in T lymphocytes

DNA-binding domain hybrid “bait” Activation-domain hybrid “prey”s Leu-protothrophy
PKCθ NH2-terminus control -
PKCθ COOH-terminus control -
PKCθ C2-like domain control -
PKCα NH2-terminus control -
PKC θ NH2-terminus Coronin 1A wt +
PKCθ COOH-terminus Coronin 1A wt -
PKCα NH2-terminus Coronin 1A wt -
PKC θ C2-like domain Coronin 1A wt ++
PKC θ NH2-terminus Coronin 1A mutant +
PKCθ COOH-terminus Coronin 1A mutant -
PKCα NH2-terminus Coronin 1A mutant -
PKCθ C2-like domain Coronin 1A mutant ++
control Coronin 1A wt -
control Coronin 1A wt -
control Coronin 1A mutant -
control Coronin 1A mutant -
  1. PKCθ and Coro1A were identified as interaction partners applying the GAL4 Two-Hybrid System. Saccharomyces cerevisiae reporter strain EGY48 was co-transformed with the bait construct encoding the NH2-terminal regulatory domain of PKCθ fused to a GAL4 DNA-binding domain and the human lymphocyte Matchmaker cDNA library. In re-transformation analysis, the NH2 and COOH domain of PKCθ were tested for interaction with truncated versions of Coro1A as indicated.