Figure 7

Akt inhibition reduces the capacity of human moDC to induce T cell responses. moDC generated in vitro with GM-CSF and IL-4 alone (4/GM) or with imatinib (3 μM) or Akt inhibitor MK2206 (300 nM) were used as stimulators in MLR with allogeneic T cells. Increasing concentration (0.0 μg/mL - 20 μg/mL) of blocking soluble recombinant T cell ligand SD-4 were added with recombinant Klotho β serving as control. T cell proliferation was measured by [³H]thymidine incorporation. CCPM = corrected counts per minute. The mean (±SD) of quadruple measurements is shown. Exemplary result from three independent experiments using different donors is presented.