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Figure 4 | Cell Communication and Signaling

Figure 4

From: Constitutive activation of oncogenic PDGFRα-mutant proteins occurring in GIST patients induces receptor mislocalisation and alters PDGFRα signalling characteristics

Figure 4

Activity-dependent mislocalisation of mutant PDGFRα. (A) 293FR-PDGFRα-GFP stable cell lines were treated with doxycycline for 14 h and subjected to confocal live cell microscopy. For figures d-f, the cells were additionally treated with 1 μM Gleevec for 14 h. (B) 293FR-PDGFRα stable cell lines were treated with doxycycline for 14 h, fixed, permeabilized and stained with DAPI (nuclear marker), GM130 (Golgi marker) and anti-PDGFRα antibody as described in the Methods section. The merged image represents PDGFRα proteins in red, the Golgi compartment in green, the nucleus in blue and PDGFRα/Golgi colocalisation in yellow.

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