Influence of EtOH and INK128 on cell cycle,
proliferation and apoptosis of DLBCL cells. Cells were treated with indicated doses of EtOH or INK128 as described in Material and Methods. (A) At 48 h after treatment, cells were stained with PI and cell cycle distribution was monitored by flow cytometry. (B) At 72 h after indicated treatments, cell numbers were counted with a hemocytometer, (C) cells were stained with Annexin V and PI, and percent of apoptotic cells was measured by flow cytometry or (D) cleavage of PARP-1 and Caspase-3 was analyzed by western blotting. β-Actin served as a loading control. The data are representative of at least three independent repeats. In the graphs (A), (B) and (C), the means and SD are shown. *p ≤ 0.05. Rap., 20 nM rapamycin.