Validation of microarray data. (A) SUDHL-2 cells were treated as described in Figure 4. Cell lysates were fractionated through sucrose gradients and EtOH- or INK128-induced changes in translational profile of selected for validation genes (and housekeeping GAPDH mRNA) were monitored by RT-qPCR analysis of RNA from each of 11 fractions. (B) Protein levels of validated genes were analyzed by western blotting. β-Actin was used as a loading control. Rap., 20 nM rapamycin. Data are representative of three independent experiments.