Figure 4

SWAP70 is required to convey vGPCR pro-angiogenic actions in endothelial cells. A-D. Parental (Par) and vGPCR-expressing SVEC (−) were stably transfected with control (shC) or two shRNAs against SWAP70 (sh1 and sh3) plasmids. A. Cells were processed for semi-quantitative RT-PCR to determine SWAP70 mRNA expression. Actin mRNA levels were detected as a control for input and equal loading. B. Permeability assays were performed as described in methods. C-D. SVEC cells were also tested for tube formation capacity (C) and sprouting ability (D). Representative images are shown. Tube length and number of branch points per field of view (FOV) were quantified in C. Number of sprouting cells and mean sprout length were determined in D. ***p < 0.001, **p < 0.01 by analysis of variance (ANOVA).