SWAP70 modulates vGPCR- and IL-8-mediated permeability through Rac activation. A. Permeability of SVEC (parental) or SVEC stably expressing vGPCR (vGPCR) that were transfected with non-silencing siRNA (nonsi) or siRNA against SWAP70 (S70si). B. Activated Rac was detected by Rac pulldown in cells with (+) and without (−) vGPCR expression following transfection with nonsi or S70si. C. Permeability of non-stimulated SVEC (control) or those stimulated with either VEGF or IL-8 was determined after transfection S70si or nonsi. D. Activated Rac was detected by Rac pulldown in cells with (+) and without (−) IL-8 stimulation following transfection with nonsi or S70si. Data are representative of three independent experiments. ***p < 0.001; **p < 0.01; ns, non significant by analysis of variance (ANOVA).