Figure 6

Radiographic EMSA dried gel, labelled with an NF-κB recognition sequence. NR8383 cells were stimulated with opsonized zymosan and exposed to clinorotation (Pipette-Clinostat) for 4 h. Nuclear lysates were incubated with ³²P labelled NF-κB recognition sequences and unravelled upon its mass. Free oligonucleotides run faster compared to NF-κB:DNA complexes. The film represents one of four individual experiments.