Figure 6
Effect of RA-PI3Kγ on CNG channel activity. HEK-293 T cells transfected with CNGA1 + empty plasmid (pCDNA3) or RA-PI3Kγ or CNGA1 + RA-PI3Kγ and channel activity was measured as a function of Ca2+ influx in response to cGMP stimulation (A). The data are mean ± SD, n = 3 independent transfections. Protein expression in cell lysates was determined by immunoblotting with anti-CNGA1 and anti-Myc antibodies (B). The RA domains of Grb14 (C), PI3Kγ-wild-type (D), and PI3Kγ-mutant (incorporation of two Glu in between Asn 299 and Gly 300; E) complete view and zoomed version are shown in each case. Panel F represents the channel activity measured as a function of Ca2+ influx in response to cGMP stimulation. Protein expression of PI3Kγ-wild-type and PI3Kγ-mutant alone or co-expressed along with CNGA1 in HEK-293 T cell lysates was determined by immunoblotting with anti-CNGA1 and anti-Myc antibodies (G).