PI3Kγ isoform predominantly associates with CNGA1. Solubilized LROS was immunoprecipitated using anti-CNGA1 antibody and the washed immunoprecipitates were incubated with either buffer or DMSO or PI3Kγ inhibitor 1 and 2 or PI3Kα inhibitor 1 and 2 for 30 min. Then, the PI3K activity assay was carried out using PI-4,5-P2 and [γ32P]ATP as substrates (A). The radioactive spots of PI-3,4,5-P3 were scraped from the TLC plate and counted using scintillation counter (B). Data are mean ± SD (n = 4). *p < 0.05, **p < 0.001 (compared to buffer and DMSO control).