PI3K interacts with CNGA1
in vivo. PI3K enzyme activity was measured in anti-CNGA1 or non-immune IgG immunoprecipitates from solubilized ROS. PI3K activity was measured using PI-4,5-P2 and [γ32P]ATP as substrates (A). The radioactive spots of PI-3,4,5-P3 were scraped from the TLC plate and counted (B). Data are mean ± SD (n = 3), *p < 0.001. Immunoblot analysis of ROS proteins from dark- and light-adapted rats with anti-CNGA1(C), p110α (D), p110β (E), p110γ (F), and anti-opsin (G) antibodies.