Figure 1

CML-Derived Exosomes Promote in Vitro and in Vivo Tumor Growth. Cell growth was evaluated by BrdU assay (a). For the experiment LAMA84 cells were incubated for 48, 72, or 96 hours, or 1 week, with increasing doses of LAMA84-exosomes (5–10–20 μg/ml). The values were plotted as a percentage of the control (untreated cells); each point represents the mean ± SD for three independent experiments. (b) The addition of 1, 5, 10, 20 or 50 μg/ml of LAMA84 exosomes increased the area of CML colonies in methylcellulose compared with controls. Asterisks indicate a statistically significant values in comparison to control (Ctrl) (*p ≤ 0.05; **p ≤ 0.01; *** p ≤ 0.001). (c) LAMA84 cells were injected subcutaneously in NOD/SCID mice, and mice were treated as described in Material and Methods. Comparison of the median tumor weight was used as an index of the proliferative efficacy of CML-derived exosomes.