Figure 5
From: The Rho GTPase RhoB regulates cadherin expression and epithelial cell-cell interaction
RhoB reduces N-cadherin levels in PC3 cells. (A) PC3 cells transfected with the indicated siRNAs were stained for F-actin and nuclei (DAPI in merge images), and with antibodies to N-cadherin. Bars 20 μm. (B) Western blot of cell lysates from PC3 cells transfected with the indicated siRNAs, and probed with the indicated antibodies. Relative levels of N-cadherin expression normalized to α-tubulin loading control are shown below the blot. (C) Quantification of N-cadherin expression. Graph shows mean expression of N-cadherin relative to siControl-1+/− s.e.m. from 4 different experiments; *p < 0.05. a.u., arbitrary units.