Figure 1

RhoB knockdown affects colony formation in DU145 cells. DU145 cells were transfected with the indicated siRNAs and analyzed after 72 h. (A) Phase contrast images of DU145 cells transfected as indicated. Bars 25 μm. (B) Representative images from Cell Profiler neighbor analysis. Cells are colored by the number of neighbors (i.e. adjacent cells), with higher numbers in warmer colors. (C) Histogram showing distribution of number of cell neighbors, determined from representative images shown in A. (D) Quantification of neighbors per cell determined from Cell Profiler neighbor analysis. Boxes of box and whisker plots show median, 25th and 75th percentile; whiskers show 95th percentile. Results are from 3 independent experiments with more than 150 cells per experiment and condition. Results from two different siRNA oligos for each gene were pooled. *p < 0.01. (E) Migration speed of DU145 cells on tissue culture-treated plastic. Cells were transfected with the indicated siRNAs. More than 50 cells per condition from 3 different experiments were tracked 58–60 h after transfection for 12–14 h. Boxes of box and whisker plots show median and 25th and 75th percentile; whiskers show 95th percentile. *p < 0.05.