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Table 1 miRNA signatures of myoblast differentiation and TNF-α or IGF1 treatment

From: TNF-α and IGF1 modify the microRNA signature in skeletal muscle cell differentiation

A

  

TLDA

Agilent

Human miRNA (hsa-miR)

 

Differentiation ΔΔCt

 

TNFα ΔΔCt

Differentiation log2

 

TNFα log2

1

 

2.0

 

−0.2

1.1

 

−1.1

133b

●●

2.3

 

−3.2

1.9

 

−2.0

198

 

1.1

 

−2.1

n.d.

 

n.d.

206

●●

2.2

 

−2.1

2.0

 

−2.1

216a-5p

●●

−4.4

 

5.5

n.d.

 

n.d.

218-5p

 

0.8

 

2.4

0.2

 

2.4

299-5p

 

2.2

 

−0.8

0.3

 

−0.2

433

●

2.7

 

−2.1

n.d.

 

n.d.

451a

 

-

 

-

−2.6

 

0.1

B

   
  

qPCR array

microarray

murine miRNA (mmu-miR)

 

Differentiation ΔΔCt

TNFα ΔΔCt

IGF1 ΔΔCt

Differentiation log2

TNFα log2

IGF1 log2

125b-2-3p

 

2.0

−0.9

−0.7

n.d.

n.d.

n.d.

129-5p

 

−2.4

−0.6

0.1

n.d.

n.d.

n.d.

133a-3p

●●

1.1

−0.3

−0.1

2.1

−0.5

0.3

133a-5p

 

-

-

-

3.4

−0.8

0.5

133b-3p

●●

2.7

−0.6

0.2

2.4

−0.5

0.4

137-3p

 

1.3

−2.0

−0.6

n.d.

n.d.

n.d.

146a-5p

 

0.0

3.6

−1.2

n.d.

n.d.

n.d.

202-3p

 

−4.1

5.6

4.4

−1.2

1.2

−0.1

206-3p

●●

2.6

−0.3

0.1

2.5

−0.3

0.5

322-3p

●

2.5

−1.3

−0.1

1.8

−1.0

0.2

330-3p

 

−0.7

−2.2

−6.0

2.7

−1.0

0.0

335-3p

●●

2.5

−0.9

0.0

2.7

−1.0

0.0

351-5p

 

2.1

−0.8

0.7

2.3

−0.9

0.4

434-5p

 

0.8

−2.1

0.3

n.d.

n.d.

n.d.

451a

 

−5.2

−4.3

1.2

n.d.

n.d.

n.d.

468-3p

 

-

-

-

−0.8

−1.4

−2.0

483-3p

●

2.6

0.4

0.2

0.6

0.4

0.3

503-3p

 

2.4

−0.8

0.3

n.d.

n.d.

n.d.

503-5p

 

1.4

−1.3

0.0

2.3

−1.7

0.1

542-3p

 

1.3

−1.5

0.2

2.6

−2.4

−0.7

  1. Differential miRNA expression is depicted as deltadelta Cq or log2 (signal intensity) for (A) human and (B) murine miRNA expression profiling by qPCR or microarrays for the effect of differentiation and TNF-α or IGF1 treatment. miRNAs with p-values < 0.05 and expression regulation > 4-fold in at least one test series are depicted. Bold numbers indicate significant (p < 0.05) and > 2-fold regulation. Bold miRNAs imply cross-platform validation: The respective miRNAs were significantly regulated in at least one of the treatment effects on both platforms (TLDA and Agilent). One black circle denotes if the miRNA is among the 25% most expressed miRNAs in at least one of the treatment groups on the qPCR array. Two black circles depict miRNAs which are among the 15% most expressed miRNAs in at least one of the treatment groups on the qPCR array. Some miRNAs could not be detected (n.d.) or were not present (dash) on one of the platforms.
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Cell Communication and Signaling

ISSN: 1478-811X