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Figure 2 | Cell Communication and Signaling

Figure 2

From: Effect of tyrosine autophosphorylation on catalytic activity and subcellular localisation of homeodomain-interacting protein kinases (HIPK)

Figure 2

Tyrosine phosphorylation of HIPK mutants. HeLa cells were transiently transfected with the indicated expression constructs for HIPK1 (A), HIPK2 (B), HIPK3 (C) or HIPK4 (D). Sodium orthovanadate (Na3VO4) was added to every second sample for 1 h before lysis. GFP fusion proteins were immunoprecipitated and analysed by immunodetection with antibodies for pTyr and GFP. The panels are representative of 2–3 independent experiments.

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