Figure 2
From: Engineering synthetic antibody binders for allosteric inhibition of prolactin receptor signaling
sAB inhibition of hPRL-R in T47D breast cancer cells. a-b Fluorescence microscopy images of cells treated with 100 nM cy5-hPRL (a) or cy5-hGH (b) in the presence of each sAB. All panels represent two merged channels; blue: DAPI nuclear stain, red: cy5. c A Stat5 phospholyration luciferase reporter assay of T47D cells in the presence or absence of hPRL and hPRL-R sABs. A sAB against bacterial maltose binding protein (MBP) [7] was used as a negative control. d Western blot detection of the time-dependent inhibition of hPRL-R signaling by hPRL-R sABs.