Figure 1

Succinate induces pathologic cardiac hypertrophy. A. Cardiac morphometric analysis after 5 days of consecutive succinate administration. Upper panels show representative images of cell width. The specimen was stained with hematoxylin and eosin (original magnification; x 100, scale bars; 50 μm). The bar graph shows quantitative analysis of cardiomyocyte diameter (n = 50, ***p < 0.001). B. The upper panels show representative images of the nuclear diameter. The bar graph summarizes data from the nuclear diameter. (n = 5, ***p < 0.001). Scale bar 6 μm. C. ANP mRNA levels in freshly isolated adult cardiomyocytes from control and succinate treated rats. D. BNP mRNA levels in freshly isolated adult cardiomyocytes from control and succinate treated rats. E. MYH7 mRNA levels in freshly isolated adult cardiomyocytes from control and succinate treated rats. F. α-SkA mRNA levels in freshly isolated adult cardiomyocytes from control and succinate treated rats. These results represent the mean ± S.E. of three separate experiments (***p < 0.001).