Figure 3
From: The structure function of the death domain of human IRAK-M
Expression and functional analysis of IRAK-M C-terminal domain mutants in 293T cells. (A) Analysis of transient expression of IRAK-M and C-terminal domain mutants by transfection in 293T cells by Western blotting performed on cell lysates with an antibody directed to full length IRAK-M. (B) Effect of IRAK-M C-terminal domain mutation on NF-κB activation by overexpression in 293T cells. (C) Effect of IRAK-M C-terminal domain mutation on A20 mRNA induction by overexpressing in 293T cells. (D) Effect of IRAK-M C-terminal domain mutation on IL-8 protein and mRNA production by overexpression in 293T cells. (B-D) Comparison to IRAK-M-DD mutants in the D19-A23 stretch. N=4, mean±SEM. *Indicates difference with WT IRAK-M P<0.05.