Figure 2
From: The structure function of the death domain of human IRAK-M
Expression and functional analysis of human IRAK-M-DD mutants in 293T cells. (A) Analysis of expression levels of IRAK-M variants by transfection in 293T cells by Western blotting performed on cell lysates with an antibody directed to the C-terminal of IRAK-M. (B) Effect of IRAK-M-DD mutations on NF-κB activation by overexpression in 293T cells. (C) Effect of IRAK-M-DD mutations on A20 mRNA induction by overexpression in 293T cells. (D) Effect of IRAK-M-DD mutations on IL-8 protein and mRNA production by overexpression in 293T cells. (B-D) N=4, mean±SEM. *Indicates difference with WT IRAK-M P<0.05. Shaded bars depict results of IRAK-M molecules with combinations of mutated residues/stretches within the same patch.