Figure 2

Brachyury mediates TGF-β1-induced EMT in HK-2 cells. (A) Immunofluorescence analysis of plakoglobin, E-cadherin and vimentin expression in pcDNA3.1 control and pcDNA3.1 Brachyury transfected HK2 cells. The green signal represents the staining of the corresponding protein. (B) Western blot analysis of plakoglobin, E-cadherin, fibronectin, α-SMA and vimentin expression in pcDNA3.1 control and pcDNA3.1 Brachyury transfected HK2 cells. A representative blot from three independent experiments is shown (Top). The histogram shows the average volume density normalized to the loading control, β-actin (Bottom) (n = 3). ^*P < 0.05 and ^**P < 0.01 compared with the pcDNA3.1 empty vector transfected cells. (C) Western blots analysis of plakoglobin, E-cadherin, fibronectin, α-SMA, vimentin and Brachyury expression in Brachyury-specific siRNA or scrambled siRNA transfected HK2 cells. A representative blot from three independent experiments is shown (Left). The histogram shows the average volume density normalized to the loading control, β-actin (Right) (n = 3). ^*P < 0.05 and ^**P < 0.01 compared with the parental cells and control cells.