Figure 1

Brachyury is induced rapidly during TGF-β1–mediated EMT. (A) qRT-PCR analysis of Brachyury of HK2 cells that were incubated with 5 ng/ml TGF-β1 for various periods. The results shown were representative of three independent experiments. The histogram showed the average volume density corrected for the loading control, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (n = 3). ^*P < 0.05 and ^**P < 0.01 compared with the control. (B) Western blotting analyses demonstrate that TGF-β1 induced Brachyury protein expression in a time-dependent manner. A representative blot from three independent experiments was shown. The histogram showed the average volume density corrected for the loading control, β-actin(n = 3). ^*P < 0.05 and ^**P < 0.01 compared with control. (C) qRT-PCR analysis of Brachyury of HK2 cells that were incubated at various concentrations of TGF-β1 for 24 h as indicated. The results shown were representative of three independent experiments. The histogram showed the average volume density corrected for the loading control, GAPDH (n = 3). ^*P < 0.05 and ^**P < 0.01 compared with control. (D) Western blotting analyses demonstrate that TGF-β1 induced brachyury protein expression at various concentrations of TGF-β1 for 24 h. A representative blot from three independent experiments was shown. The histogram showed the average volume density corrected for the loading control, β-actin (n = 3). ^*P < 0.05 and ^**P < 0.01 compared with control.