Figure 9
From: CANDLES, an assay for monitoring GPCR induced cAMP generation in cell cultures
Adapting GS-293 cells to grow in RPMI 1640 and McCoy’s 5A media. Both GS-293 and FSHR-293 cells were step-wise adapted to grow in either McCoy’s 5A or RPMI 1640 media. After adapting cells, co-cultures of GS-293 (50,000 cells) and FSHR-293 (50,000 cells) were grown in either medium. (A and B) The CANDLES assay, performed after adapting the cells, can detect cAMP from co-cultured donor cells. Data represented as mean of triplicates for one representative experiment (± SEM) with at least three independent repeats.