Figure 8

Monitoring cAMP generation in primary cells with GS-293 cells. (A) Mouse granulosa cells were co-cultured with GS-293 (100,000 cells). GS-293 detected cAMP generated by rLH (100 ng/ml) stimulation of LHCGR in granulosa cells. (B) Rat cortical neurons were co-cultured with GS-293 (100,000 cells) and stimulated with epinephrine or glutamate. Epinephrine led to generation of cAMP, while glutamate resulted in no response. (A and B) Data represented as mean of duplicates for one representative experiment (± SD) with at least three independent repeats. (C) Rat cortical neurons were co-cultured with GS-293 (100,000 cells) and stimulated with isoproterenol (100 nM), epinephrine (100 nM) and salbutamol (100 nM) to compare their different kinetic responses upon stimulation of adrenergic receptors. (D) GS-293 cells also express adrenergic receptors as can be seen in response to isoproterenol, epinephrine and salbutamol stimulation, although the response to stimulation is minimal in comparison to cortical neurons (compare the Y-axis with Figure 8C). (C and D) Data represented as mean of triplicates for one representative experiment (± SEM) with at least three independent repeats.