Figure 4
From: CANDLES, an assay for monitoring GPCR induced cAMP generation in cell cultures
Optimization of the ratio of sensor (GS-293) to donor cells for maximal luminescence (cAMP generation). (A) Cell seeding density of GS-293 cells: KK-1 cells in increasing ratio from 1:1 to 1:4 (25,000 cells of each for 1:1 ratio). (B) Cell seeding density of GS-293: FSHR-293 cells in increasing ratio from 1:1 to 1:4 (25,000 cells of each for 1:1 ratio). (A and B) An increased luminescent signal [proportional to cAMP production upon rLH (100 ng/ml) or rFSH (200 mIU/ml) stimulation] was detected with increasing number of donor cells (KK-1 or FSHR-293). (C) Cell seeding density of FSHR-293: GS-293 cells in increasing ratios from 1:1 to 1:4 (25,000 cells of each for 1:1 ratio) and a decrease in luminescent signal was observed for an increasing number of GS-293 cells. (A, B and C) Data represented as mean of triplicates for one representative experiment (± SEM; positive direction) with at least three independent repeats.