Figure 2From: CANDLES, an assay for monitoring GPCR induced cAMP generation in cell culturesCANDLES Assay protocol. The CANDLES assay involves co-culture of sensor cells (GS-293 or EPAC-293) with donor cells (primary cells or cell lines). DMEM complete medium is replaced with freshly prepared assay medium and cells are pre-incubated (in dark for GS-293 cells) before luminescence/ FRET measurements can be read upon ligand stimulation.Back to article page