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Figure 2 | Cell Communication and Signaling

Figure 2

From: Alteration in mitochondrial Ca2+ uptake disrupts insulin signaling in hypertrophic cardiomyocytes

Figure 2

Reduction in insulin-dependent mitochondrial Ca2+uptake in NE-induced hypertrophic cardiomyocytes. Representative measures of fluorescence-associated insulin-induced Ca2+ signals in cardiomyocytes pre-treated with (gray line) or without (Ctrl, black line) the respective hypertrophic inducers for 24 h. Insulin (100 nM) was added at 100 s. A) Mitochondrial Ca2+ signals in cardiomyocytes treated with NE 10 μM for 24 h. B) Cytoplasmic Ca2+ signals in cardiomyocytes treated with NE 10 μM for 24 h. C) Mitochondrial Ca2+ signals in cardiomyocytes treated with IGF-1 100 nM for 24 h. D) Area under the curve of mitochondrial Ca2+ signals of control and IGF-1 and NE treated cardiomyocytes after insulin stimulation between 100 and 300 s. The results are representative of 3 independent experiments (N = 3), where 10–20 cells were analysed. Data are expressed as mean ± SEM, *P < 0.05 vs. Ctrl. E) Measurement of histamine-induced mitochondrial Ca2+ signal in cardiomyocytes treated with NE 10 μM for 24 h. Histamine (100 mM) was added at 100 s. F) Area under the curve of mitochondrial Ca2+ signals of control and NE treated cardiomyocytes after histamine stimulation between 100 and 300 s. The results are representative of 3 independent experiments (N = 3), where 10–20 cells were analysed. Data are expressed as mean ± SEM, *P < 0.05 vs. Ctrl.

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