Figure 2
From: MicroRNA-188 suppresses G1/S transition by targeting multiple cyclin/CDK complexes
MiR-188 downregulates multiple genes related to G 1 /S transition. (A) CCND1, CCND3, CDK4, CCNA2, CDK2 and CCNE1 are predicted to be miR-188 target genes. (B) qRT-PCR analysis of mRNA expression levels of miR-188 targets in CNE cells transfected with miR-NC or miR-188. Data are presented as means ± SD of three independent experiments (Student t test, *p < 0.05, **p < 0.01, ***p < 0.001). (C) Overexpression of miR-188 downregulates the protein expression of cyclin E1, CDK2, cyclin A2, cyclin D1, cyclin D3 and CDK4. (C1, stable clone 1; C2, stable clone 2). All western blots were performed at least three times. (D) Western blot analysis of cyclin E1, CDK2, cyclin A2, cyclin D1, cyclin D3 and GAPDH in CNE cells transfected with Ant-188 or Ant-NC. All western blots performed at least three times. (E) Inverse correlation between miR-188 and its targets in NPC tissues. The relative levels of miR-188, CCND1, CCND3, CDK4, CCNA2, CDK2 and CCNE1 were quantified using qRT-PCR.