Figure 5

LPS-induced activation of hBMVEC basolateral iron efflux through modulation of hBMVEC interleukins with subsequent C6 glioma cell sCp gene activation. (A) LPS (100 μg/mL) was added to hBMVEC for the times indicated before total hBMVEC RNA was collected and assayed for IL-1β and IL-6 transcript abundance via qPCR. (B) Total RNA was isolated from C6 glioma cells seeded distal to hBMVEC incubated with or without the addition of LPS (100 μg/mL) to the apical chamber for 24 h. Soluble Cp transcript abundance was assessed via qPCR. (C) C6 glioma cells were incubated alone or distal to hBMVEC for 20 h prior to the addition of LPS (100 μg/mL) to the apical chamber and IRAP (1 μg/mL) and/or SC144 (20 μM) to the basal chamber for an additional 4 h as indicated. Total C6 glioma RNA was isolated and assayed for sCp transcript abundance via qPCR. Significance of the data was determined using either a paired t-test or one-way ANOVA statistical analyses. ***P < 0.001. Data are represented as means ± s.d. (n = 3, technical replicates).