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Figure 4 | Cell Communication and Signaling

Figure 4

From: Activation of C6 glioblastoma cell ceruloplasmin expression by neighboring human brain endothelia-derived interleukins in an in vitro blood-brain barrier model system

Figure 4

Increased C6 glioma sCp expression enhances hBMVEC59Fe-efflux. (A) Illustration of in vitro co-culture setup in transwells. In all cases the apical chamber contained RPMI1640 plus serum while the basolateral chamber was devoid of serum. (B) hBMVEC were loaded for 24 h with 59FeII-citrate plus ascorbate and then iron efflux assays were performed. The percent loss of cell-associated 59Fe from hBMVEC after 24 h relative to t = 0 h was monitored. Efflux buffer was basolateral media conditioned for 24 h with the cellular orientations depicted in A. Significance of the data was determined using one-way ANOVA statistical analysis of the variance parameters. *P < 0.05, **P < 0.01, ***P < 0.001. Data are represented as means ± s.d. (n = 8, experimental replicates).

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