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Figure 2 | Cell Communication and Signaling

Figure 2

From: Activation of C6 glioblastoma cell ceruloplasmin expression by neighboring human brain endothelia-derived interleukins in an in vitro blood-brain barrier model system

Figure 2

Interleukin-1β and interleukin-6 are expressed by hBMVEC. (A) RT-PCR of total RNA from hBMVEC examining the presence of IL-6 and IL-1β transcript. (B) Immunoblot of basal hBMVEC-conditioned media (Basal ECM) probing for IL-1β (lanes 1 (20 μL sample loaded) and 2 (10 μL sample loaded)) and IL-6 (lane 3). (C-D) qPCR of hBMVEC IL-6 (C) or IL-1β (D) transcripts from total RNA isolated from hBMVEC grown either alone (EC) or distal to C6 glioma cells (EC/-/C6). A series of paired t-tests were used to determine the significance of the data. *P < 0.05. Data are represented as means ± s.d. (n = 3, technical replicates).

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