Figure 4
HMC-1 exosomes transfer KIT protein to A549 cells. Eighty microgram of HMC-1 exosomes were added per 4 ×105 A549 cells and incubated at 37°C for 24 hours for the Western blot experiments (A) and for 30 minutes - 48 hours for the RT-PCR experiments (B). A) Western blot confirmed the presence of the KIT protein in HMC-1 exosomes, but not in the A549 cells. SCF expression was detected in A549 cell but not in the HMC-1 exosomes. SCF and KIT were both detected in A549 cells 24 hours after the addition of HMC-1 exosomes. B) RT-PCR showed that c-KIT mRNA was present in HMC-1 cells, whereas it was absent in both HMC-1 exosomes and A549 cells. GAPDH mRNAs were detected as positive control and were detected in all samples except in HMC-1 exosomes.