Figure 1

Identification and characterization of HMC-1 exosomes. Exosomes were isolated using differential centrifugation. A) The electron micrographs of the exosomes revealed rounded structures with a size of approximately 30-100 nm. Arrows indicate 10-nm gold-labeled anti-CD63. The scale bar indicates 200 nm. B) Western blot analysis of exosomes derived from HMC-1 cells supernatant shows presence of the common exosome proteins, CD81 and TSG101, but absence of the endoplasmic reticulum protein, calnexin. Also control exosomes isolated from NIH 3T3 and HEK 293 cells were positive for TSG101 and CD81, but negative for calnexin. Cells were used as a control.