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Figure 3 | Cell Communication and Signaling

Figure 3

From: Role of gamma-secretase in human umbilical-cord derived mesenchymal stem cell mediated suppression of NK cell cytotoxicity

Figure 3

Mechanism of MSC-mediated suppression of NK cell degranulation. A: NK cells were cultured in normal medium or in MSC cm or in NK-MSC cm. CD107a degranulation assay was performed with K562 as target cells. Representative dot plots of the alterations in NK cell degranulation are shown (n = 3). B: NK cell-mediated cytotoxicity was determined by chromium release assay in different conditioned media (n = 3). C: UC-MSCs were cultured overnight with or without NK cells. Intracellular staining for COX-2 was performed and analysed by flow cytometry. One representative figure and bar graphs (n = 5) depicting COX-2 upregulation in UC-MSCs cultured with NK cells in comparison with COX-2 expression in "unprimed" UC-MSCs is shown. D: NK cells were cultured with or without MSCs or co-cultured in presence of NS-398 or DMSO. CD107a degranulation assay was performed with K562 target cells. The bars represent the effect of COX-2 inhibition on degranulative capacity of NK cells (n = 5). E: NK cells from four different donors were cultured for 16 hours and culture supernatants were collected. The bars represent the IL-1β levels measured in the supernatants (n = 4). F: MSCs were co-cultured with NK cells in presence of IL-1β neutralising antibody or matched isotype antibody. The bars illustrate the effect of IL-1β blocking on COX-2 expression (n = 4).

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