Figure 5From: MiR200-upregulated Vasohibin 2 promotes the malignant transformation of tumors by inducing epithelial-mesenchymal transition in hepatocellular carcinomaThe effect of VASH2 expression on cell apoptosis and SP cell proportion. (A) Flow cytometry to determine the apoptosis rate in HepG2 cells with different levels of VASH2 expression after CDDP treatment. Line graph of the apoptosis rate (*represents p < 0.05 when compared to HepG2-wt). qPCR of ABCC1 (B) and Bcl-2 (C) in HepG2 cells with different levels of VASH2 expression. (D) Western blot of ABCC1 and Bcl-2 in HepG2 cells with different levels of VASH2 expression. (E) Promoter luciferase reporter assay for regulation of VASH2 on ABCC1 and BCL2. (F) SP cell proportion of HepG2 cells with different levels of VASH2 expression. (G) SP cell proportion of Hep3B cells with different levels of VASH2 expression. (*represents p < 0.05 when compared to the control group).Back to article page