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Figure 2 | Cell Communication and Signaling

Figure 2

From: Claudin-4 controls the receptor tyrosine kinase EphA2 pro-oncogenic switch through ?-catenin

Figure 2

Effect of siRNA-mediated knockdown of EphA2 on migration of 2008/SCB and CLDN4KD cells. (A) Relative motility was determined by the ability of 2008/SCB and CLDN4KD cells with or without knockdown of EphA2 expression to close a wound made by creating a scratch through a lawn of confluent cells. Cell images were taken at 0 and 8 h after the scratch. The histogram shows quantification of the migration speed from two independent experiments each performed with triplicate cultures. Migration distance (?m) travelled 8 h after the scratch was determined using Slidebook (v5.0) software and average motile speed (?m/min) was calculated. Values are the mean ± SEM; **p?<?0.01 versus 2008/SCB/siRNA-Control, ##p?<?0.01 versus CLDN4KD/siRNA-Control. (B) Expression of CLDN4 and EphA2 in the indicated four cell lines by Western blot analysis. The histogram summarizes the results of 3 independent experiments expressed as the fold change relative to that in the scrambled siRNA control 2008/SCB cells after normalization to ?-actin. Results are reported as mean ± SEM (n = 3).

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