Nuclear clustering of autophagosomes, lysosomes and autolysosomes under ND conditions. MCF7 cells stably expressing GFP-Rab7 and mCherry-LC3, submitted to 3 hours of ND. N designates the bounded nuclear region, and P corresponds to the periphery of the cell. The M (perinuclear) region was obtained by partitioning the cell at an equal distance between the N and P lines. Numbers are cell identifiers. Scale bar, 10 μm.