Bafilomycin A1 decreases mTOR activity under FM and ND conditions. HeLa cells were submitted to FM and ND conditions, in the absence and presence of BAF (100 nM). Western blot analysis was performed with protein samples taken during time periods of 0-3 hours (FM) and 0-1 hours (ND). Antibodies against 4EBP1 and phosphorylated 4EBP (p-4EBP1) were used to detect mTOR activity. An antibody against GAPDH was used as loading control.